Inicio \ Investigación \ Publicaciones científicas \


Drug susceptibility of Spanish Mycobacterium tuberculosis complex isolates from animals

Artículo de investigación publicado en Tuberculosis (Edinburgh, Scotland)

1 de noviembre de 2007

Mycobacterium bovis and Mycobacterium caprae are zoonotic bacteria that cause tuberculosis with several clinical manifestations. We have evaluated the susceptibility to anti-tuberculosis drugs of a panel of Spanish isolates of animal origin. The analysis of the sequence of the main genes involved in resistance was performed in 41 M. bovis and five M. caprae. The katG, inhA, rpsL, embB and gyrA genes had single nucleotide polymorphisms, not previously described in other organisms of the complex. Thirty-two M. bovis and three M. caprae isolates were tested for susceptibility to isoniazid (INH), rifampin, streptomycin, ethambutol, and ofloxacin using the standard proportion method. The results revealed that the isolates were sensitive to the five drugs. However, interference caused by sodium pyruvate in the INH test was detected: 94.3% grew at 0.2 microg INH/ml and 68.6% grew at 1 microg INH/ml. In the medium without pyruvate, 34.3% of the isolates did not grow whereas growth of the others was poor and slow. Nine M. bovis isolates were also tested by ESP Culture System II test and were sensitive to INH. The susceptibility of M. bovis to INH cannot be reliably determined using the standard proportion method due to the M. bovis growth requirements and the interference of pyruvate with INH




Romero B., Aranaz A., Bezos J., Alvarez J., de Juan L., Tariq-Javed M., Mateos A., Gomez-Mampaso E. y Dominguez L..




Ver artículo
Drug susceptibility of Spanish Mycobacterium tuberculosis complex isolates from animals

Ver en NLM PubMed
Drug susceptibility of Spanish Mycobacterium tuberculosis complex isolates from animals



Participantes:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Dept Veterinary Patholgy. University of Agriculture Faisalabad (UAF).

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Comunidad de MadridServicio de Microbiología. Hospital Universitario Ramón y Cajal. Salud Madrid. Comunidad de Madrid.







Tuberculosis (Edinburgh, Scotland)
FACTOR YEAR Q
2.454 2007

NLMID: 9885624

PMID: 17900988

ISSN: 1472-9792



TÍTULO: Drug susceptibility of Spanish Mycobacterium tuberculosis complex isolates from animals


REVISTA: Tuberculosis (Edinb)


NUMERACIÓN: 87(6):565-71


AÑO: 2007


EDITORIAL: Churchill Livingstone


AUTORES: Romero B., Aranaz A., Bezos J., Alvarez J., de Juan L., Tariq-Javed M., Mateos A., Gomez-Mampaso E. and Dominguez L..


First
Beatriz Romero Martínez
3rd
Javier Bezos Garrido
4th
Julio Álvarez Sánchez
5th
Lucía de Juan Ferré
Last
Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1016/j.tube.2007.08.004


CITA ESTA PUBLICACIÓN:

Romero B., Aranaz A., Bezos J., Alvarez J., de Juan L., Tariq-Javed M., Mateos A., Gomez-Mampaso E. y Dominguez L. Drug susceptibility of Spanish Mycobacterium tuberculosis complex isolates from animals. Tuberculosis (Edinburgh, Scotland). 87(6):565-71. 2007. (A). ISSN: 1472-9792. DOI: 10.1016/j.tube.2007.08.004


SERVICIOS: