Frontiers in veterinary science publica este artículo de investigación

24 de julio de 2025

Animal tuberculosis (TB) affects a wide range of domestic species, including goats. TB eradication programs in goats are based on cell-based techniques such as the single and comparative intradermal tuberculin test (SITT and CITT, respectively). In recent years, an ELISA technique based on the P22 protein complex (P22 ELISA), has emerged as a valuable tool for TB diagnosis. The aim of the study was to evaluate the performance of the P22 ELISA in the context of a caprine TB eradication program using serum, individual milk and bulk tank milk (BTM) samples in order to define its usefulness in classifying herds compared to SITT and CITT. Samples from 53 herds categorized based on the detection of CITT reactors (16 high-risk herds, with one or more CITT reactors, and 37 low-risk herds, with only CITT-negative goats) were analyzed. Reactors in the P22 ELISA were detected in a higher number of high-risk herds using both serum (87.5%) and individual milk (81.3%) compared to SITT (75.0%) and CITT (31.3%), while the use of BTM led to the detection of 33.3% of the herds. Individual apparent prevalence was higher using the P22 ELISA in both serum (11.0%) and milk (15.0%) compared to the SITT (6.8%) and CITT (2.5%), with also a significantly (p?

Velasco-Reinaldos C., Ortega J., Alvarez J., Infantes-Lorenzo JA., Moreno JC., Sanz C., Romero B., de Juan L., Dominguez L., Dominguez M., Moreno I., Roy A. y Bezos J..

Field evaluation of the P22 ELISA for diagnosis of caprine tuberculosis in an endemic area

Field evaluation of the P22 ELISA for diagnosis of caprine tuberculosis in an endemic area

	Servicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).
	Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).
	Laboratorio de Referencia e Investigación en Resistencia a Antibióticos e Infecciones Relacionadas con la Asistencia Sanitaria. Servicio de Inmunología. Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).
	Dirección General de Agricultura y Ganadería. Consejería de Agricultura, Desarrollo Rural, Medio Ambiente y Energía. Junta de Extremadura.
	Centro Nacional de Epidemiología (CNE). Instituto de Salud Carlos III (ISCIII).