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Single-nucleotide polymorphism in two representative multidrug-resistant Mycobacterium bovis isolates collected from patients in a Spanish hospital harboring a human infection outbreak

Journal of Clinical Microbiology publica este artículo de investigación

1 de febrero de 2008

Mycobacterium bovis is the etiological agent of tuberculosis in domestic and wild animals. Its involvement as a human pathogen has been highlighted again with the recent descriptions of transmission through dairy products (18), reactivation or primary infection in human immunodeficiency virus-infected patients (5), and association with meat industry workers, animal keepers, or hunters (3). Strains resistant to antituberculous drugs (M. bovis is naturally resistant to pyrazinamide) pose an additional risk (2). Several studies have demonstrated that mutations in target genes are associated with resistance to antituberculous drugs (4, 7, 10, 11, 16). However, most of them have been developed in Mycobacterium tuberculosis strains and limited data are available regarding M. bovis isolates.
The aim of this study was to characterize by sequencing the main genes involved in antibiotic resistance in two multidrug-resistant (MDR) M. bovis isolates in a human outbreak detected in a hospital in Madrid that subsequently spread to several countries (5, 6, 15). The isolates were resistant to 11 drugs, but only their rpoB and katG genes have been analyzed so far (1, 14). We studied the first (93/R1) and last (95/R4) M. bovis isolates of this nosocomial outbreak, characterized by spoligotyping as SB0426 (hexacode 63-5F-5E-7F-FF-60 in the database at www.mbovis.org) (1, 13).

Several genes involved in resistance to isoniazid (katG, ahpC, inhA, and the oxyR-ahpC intergenic region), rifampin (rpoB), streptomycin (rrs, rpsL), ethambutol (embB), and quinolones (gyrA) were studied. These genes, or fragments of genes, were amplified and sequenced as previously described (12).

The sequence analysis revealed polymorphisms in five (ahpC, rpoB, rpsL, embB, and gyrA) out of nine analyzed genes (Table 1). Nucleotide substitutions in four genes cause a change in the encoded amino acid. Two additional synonymous mutations in ahpC and rpsL differentiated the first and last isolates from the outbreak.




Romero B., Aranaz A., Bezos J., Alvarez J., de Juan L., Mateos A., Gomez-Mampaso E. y Dominguez L..




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Single-nucleotide polymorphism in two representative multidrug-resistant Mycobacterium bovis isolates collected from patients in a Spanish hospital harboring a human infection outbreak

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Single-nucleotide polymorphism in two representative multidrug-resistant Mycobacterium bovis isolates collected from patients in a Spanish hospital harboring a human infection outbreak


Participantes:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Comunidad de MadridServicio de Microbiología. Hospital Universitario Ramón y Cajal. Salud Madrid. Comunidad de Madrid.







Journal of Clinical Microbiology
FACTOR YEAR Q
3.945 2008

NLMID: 7505564

PMID: 18094132

ISSN: 0095-1137



TÍTULO: Single-nucleotide polymorphism in two representative multidrug-resistant Mycobacterium bovis isolates collected from patients in a Spanish hospital harboring a human infection outbreak


REVISTA: J. Clin. Microbiol.


NUMERACIÓN: 46(2):826-7


AÑO: 2008


EDITORIAL: American Society for Microbiology


AUTORES: Romero B., Aranaz A., Bezos J., Alvarez J., de Juan L., Mateos A., Gomez-Mampaso E. and Dominguez L..


PARTICIPANTES VISAVET


First
Beatriz Romero Martínez
3rd
Javier Bezos Garrido
4th
Julio Álvarez Sánchez
5th
Lucía de Juan Ferré
Last
Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1128/JCM.00193-07


CITA ESTA PUBLICACIÓN:

Romero B., Aranaz A., Bezos J., Alvarez J., de Juan L., Mateos A., Gomez-Mampaso E. y Dominguez L. Single-nucleotide polymorphism in two representative multidrug-resistant Mycobacterium bovis isolates collected from patients in a Spanish hospital harboring a human infection outbreak. Journal of Clinical Microbiology. 46(2):826-7. 2008. (A). ISSN: 0095-1137. DOI: 10.1128/JCM.00193-07


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