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Study of peripheral blood cell populations involved in the immune response of goats naturally infected with Mycobacterium caprae

Investigación publicada en Research in Veterinary Science

1 de agosto de 2012

Tuberculosis in goats caused by Mycobacterium bovis and Mycobacterium caprae has noteworthy sanitary and economic implications. Current diagnostic assays are based on cellular immunity and although they have demonstrated a high sensitivity, some animals remain undetected. In the present study, flow cytometry has been used to determine changes in CD4+, CD8+ and CD25+ T cell populations in peripheral blood from naturally infected goats. Proportion of lymphocytes producing PPD-specific interferon-gamma (IFN-γ) was calculated and an ELISA for detection of PPD-specific IFN-γ was performed to measure the cytokine in plasma. The infected goats showed percentages of CD4+ T cells between 27.31% and 47.23% and there were not significant differences (p=0.113) with the non-infected control goats although the mean percentage was lower in this group. Regarding CD8+ T cells, a higher percentage was observed in healthy goats compared to controls (p=0.081). The mean percentage of lymphocytes expressing CD25 without antigen stimulation (30.65±3.91) was higher in lesion and/or culture-positive animals than in the controls (21.84±1.21; p=0.053). The percentage of CD4+/IFN+ T cell population stimulated with bovine PPD was a reliable marker of infection, since the mean percentage in the infected goats was significantly higher than in the controls (p<0.05). Tuberculosis in goats caused by M. caprae induced changes in cellular populations similar to those described for M. bovis in cattle




Bezos J., Alvarez J., Moreno I., de Juan L., Romero B., Rodriguez-Campos S., Dominguez M., Torano A., Mateos A., Dominguez L. y Aranaz A.




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Study of peripheral blood cell populations involved in the immune response of goats naturally infected with Mycobacterium caprae

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Study of peripheral blood cell populations involved in the immune response of goats naturally infected with Mycobacterium caprae


Participantes:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Instituto de Salud Carlos IIIServicio de Inmunología. Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).

Gobierno de Castilla-La ManchaInstituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).







Research in Veterinary Science
FACTOR YEAR Q
1.774 2012

NLMID: 401300

PMID: 21689835

ISSN: 0034-5288



TÍTULO: Study of peripheral blood cell populations involved in the immune response of goats naturally infected with Mycobacterium caprae


REVISTA: Res. Vet. Sci.


NUMERACIÓN: 93(1):163-7


AÑO: 2012


EDITORIAL: Elsevier


AUTORES: Bezos J., Alvarez J., Moreno I., de Juan L., Romero B., Rodriguez-Campos S., Dominguez M., Torano A., Mateos A., Dominguez L. and Aranaz A.


PARTICIPANTES VISAVET


First
Javier Bezos Garrido
2nd
Julio Álvarez Sánchez
4th
Lucía de Juan Ferré
5th
Beatriz Romero Martínez
10th
Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1016/j.rvsc.2011.05.020


CITA ESTA PUBLICACIÓN:

Bezos J., Alvarez J., Moreno I., de Juan L., Romero B., Rodriguez-Campos S., Dominguez M., Torano A., Mateos A., Dominguez L. y Aranaz A. Study of peripheral blood cell populations involved in the immune response of goats naturally infected with Mycobacterium caprae. Research in Veterinary Science. 93(1):163-7. 2012. (A). ISSN: 0034-5288. DOI: 10.1016/j.rvsc.2011.05.020


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