Last SCI scientific publications
Neurobrucellosis (Brucella ceti) in striped dolphins (Stenella coeruleoalba): Immunohistochemical studies on immune response and neuroinflammation
Veterinary Pathology 1-11.
Neurobrucellosis is a shared condition of cetaceans and humans. However, the pathogenesis and immune response in cetacean neurobrucellosis has not been extensively studied. In this multicentric investigation, 21 striped dolphin (Stenella coeruleoalba) neurobrucellosis (Brucella ceti) cases diagnosed over a 10-year period (2012-2022) were retrospectively evaluated. For each case, morphological changes were assessed by evaluating 21 histological parameters. Furthermore, the immunohistochemical expression of Brucella antigen, glial fibrillary acid protein (GFAP), and a selection of inflammatory cell (IBA-1, CD3, and CD20) and cytokine (tumor necrosis factor-alpha [TNF-α], interferon-gamma [IFN-γ], interleukin [IL]-1β, IL-2, and … Leer más
Rebollada A., Giorda F., Pumarola M., Martino L., Gómez-Buendía A., Romani-Cremaschi U., Casalone C., Mattioda V., Di Nocera F., Lucifora G., Petrella A., Dominguez L., Domingo M., Grattarola C. and Rodriguez-Bertos A..
Detection of equine herpesvirus-1 (EHV-1) in urine samples during outbreaks of equine herpesvirus myeloencephalopathy
Equine Veterinary Journal 56(3):456-463.
Background: Real-time PCR is the diagnostic technique of choice for the diagnosis and control of equine herpesvirus-1 (EHV-1) in an outbreak setting. The presence of EHV-1 in nasal swabs (NS), whole blood, brain and spinal cord samples has been extensively described; however, there are no reports on the excretion of EHV-1 in urine, its DNA detection patterns, and the role of urine in viral spread during an outbreak.
Objectives: To determine the presence of EHV-1 DNA in urine during natural infection and to compare the DNA detection patterns of EHV-1 in urine, buffy coat (BC) and NS.
Study design: Descriptive study of natural infection.
Methods: Urine and whole blood/NS samples were collected at different time points dur… Leer más
Velloso-Alvarez A., Jose-Cunilleras E., Dorrego-Rodriguez A., Santiago-Llorente I., de las Cuesta-Torrado M., Troya-Portillo L., Rivera B., Vitale V., de Juan L. and Cruz F..
Active surveillance of antimicrobial resistance in companion animals: A pilot study in a Spanish Veterinary Teaching Hospital
Comparative Immunology, Microbiology And Infectious Diseases 108:102169.
The role of small animal veterinary hospitals in the onset and dissemination of antimicrobial-resistant organisms (AMROs) is still not clear, and the implementation of an internal surveillance systems is a cost-effective tool to better understand their impact. The aim of this study was to describe a pilot program of active surveillance in a Spanish Veterinary Teaching Hospital, developed to estimate the detection frequency of AMROs in the commensal flora of patients and in the environment. Surveillance was focused on Methicillin-resistant Staphylococci (MRS), third generation cephalosporins resistant gram-negative bacteria (3GCR-GNB), and carbapenems-resistant gram-negative bacteria (CR-GNB). Oral and perirectal swabs were collected in the … Leer más
Scarpellini R., Leal Velez de Mendizábal L., Quevedo-Caraballo S., Blanco JL., Garcia ME., Perez-Sancho M., Portero-Fuentes M., Penelo S., Esposito E., Mondo E. and Piva S.
Forensic findings in urban red fox mortality in the metropolitan area of Madrid, 2014-2022
Journal Of Veterinary Diagnostic Investigation 1-5.
The expanding presence of red foxes (Vulpes vulpes) in urban and suburban regions could potentially lead to increased instances of human aggression towards this species. We studied 10 deceased red foxes that were submitted by law enforcement agencies in the metropolitan area of Madrid in 2014-2022 because of suspected abuse. Forensic autopsies were performed to establish the cause and manner of death. In 4 of the 10 cases, the cause of death was deemed unnatural, involving blunt-force trauma (n = 2), asphyxia resulting from hanging (n = 1), and firearm injury (n = 1). Among the remaining cases, most had succumbed to natural causes (n = 4), often marked by severe emaciation and a high burden of parasites, primarily Sarcoptes scabiei. In 2 ca… Leer más
Rebollada A., Barcena C., Porras N., Dominguez L. and Rodriguez-Bertos A..
Tertiary lymphoid organs in wild boar exposed to a low-virulent isolate of African swine fever virus
The Veterinary Quarterly 44(1):1-13.
Despite the great interest in the development of a vaccine against African swine fever (ASF) in wild boar, the immunological mechanisms that induce animal protection are still unknown. For this purpose, tertiary lymphoid organs (TLOs) of wild boar were characterised and compared with mucosa-associated lymphoid tissues (MALTs) by histopathology, histomorphometry and immunohistochemistry (CD3, CD79, PAX5, LYVE1, fibronectin). In addition, real-time polymerase chain reaction (qPCR) and immunohistochemistry (p72) were used to evaluate the presence of ASF virus (ASFV) in blood and tissues samples, respectively. TLOs were observed in animals infected with a low-virulent ASFV isolate (LVI), animals co-infected with low and high-virulent ASFV isola… Leer más
Porras N., Sanchez-Vizcaino JM., Rodriguez-Bertos A., Kosowska A. and Barasona JA..
Eco-Sanitary Regionalization of Wild Boar (Sus scrofa) in the Western Palearctic Realm as a Tool for the Stewardship of African Swine Fever
Transboundary And Emerging Diseases 8080496.
African swine fever (ASF) is a viral hemorrhagic disease affecting domestic and wild suids, with catastrophic socioeconomic impact worldwide. In the European scenario, wild boar (Sus scrofa) actively contributes to the maintenance and spread of the disease. In this study, we applied a multivariate clustering method to define ecoregions in the western Palearctic realm based on ecological and sanitary aspects of ASF in wild boar. Beforehand, we performed a principal components analysis for the eight selected variables. An analysis of the countries was carried out in terms of the extent of ecoregions and ASF notifications. After clustering, we identified nine eco-regions that showed important differences based on the used eco-sanitary variable… Leer más
Aguilar-Vega C., Munoz-Perez C., Sanchez-Vizcaino JM., Martinez-Aviles M. and Bosch J.
Molecular detection of herpesvirus in a skin lesion of a humpback whale (Megaptera novaeangliae) from the Western Mediterranean Sea
European Journal Of Wildlife Research 70:31.
Herpesvirus has the potential to infect a wide variety of animal species. In cetaceans, Alpha- and/or Gammaherpesvirinae have been identified in eight families of odontocetes, and one family of mysticetes. In May 2022, an adult humpback whale (Megaptera novaeangliae) was found stranded in Valencia, Spain. The whale was emaciated, in poor body condition, with multiple lacerations on the dorsal fin and a high number of epibionts of the Cyamidae family, known as whale lice. The individual had been previously released from a ghost net entanglement 5 days before becoming stranded. In a closer examination, various skin lesions were observed, including chronic, proliferative, and erosive dermatitis and a large ulcer extending to the deep dermis. A… Leer más
Vargas-Castro I., Crespo-Picazo JL., Jimenez-Martinez MA., Munoz-Baquero M., Marco-Cabedo V., Garcia-Parraga D. and Sanchez-Vizcaino JM..
Pancreatic adenocarcinoma treated with surgical resection, toceranib phosphate and firocoxib in a dog: a case report
Veterinary Research Communications 1-7.
Exocrine pancreatic carcinomas are rarely reported in dogs. A ductal pancreatic adenocarcinoma in a 10-year-old intact beagle is described in this report. The diagnosis was made based on clinical signs, imaging (abdominal ultrasound and CT scan) and histopathology. Treatment consisted of partial right lobe pancreatectomy followed by adjuvant therapy with toceranib phosphate (Palladia®) and firocoxib (Previcox®) for six months. The treatment was well tolerated, and the survival time was 445 days. To our knowledge, this is the longest survival reported in the literature for a dog diagnosed with exocrine pancreatic adenocarcinoma. The results described here may contribute to provide a better understanding about this neoplasia and potential tre… Leer más
Fuertes-Recuero M., Vazquez-Fernandez E., Lizasoain-Sanz G., Arroba-Alonso A., Sanchez-Lopez A., Martinez-de Merlo E., Suarez-Redondo M. and Ortiz-Diez G.
Application of machine learning with large-scale data for an effective vaccination against classical swine fever for wild boar in Japan
Scientific Reports 14(1):5312.
Classical swine fever has been spreading across the country since its re-emergence in Japan in 2018. Gifu Prefecture has been working diligently to control the disease through the oral vaccine dissemination targeting wild boars. Although vaccines were sprayed at 14,000 locations between 2019 and 2020, vaccine ingestion by wild boars was only confirmed at 30% of the locations. Here, we predicted the vaccine ingestion rate at each point by Random Forest modeling based on vaccine dissemination data and created prediction surfaces for the probability of vaccine ingestion by wild boar using spatial interpolation techniques. Consequently, the distance from the vaccination point to the water source was the most important variable, followed by elev… Leer más
Ito S., Aguilar-Vega C., Bosch J., Isoda N. and Sanchez-Vizcaino JM..
Evaluation of the Effect of a Recent Comparative Intradermal Tuberculin Test on the Humoral Diagnosis of Paratuberculosis Using Serum and Milk Samples from Goats
Veterinary Sciences 11(3):105.
Paratuberculosis (PTB) and tuberculosis (TB) are two mycobacterial diseases with a severe economic and health impact on domestic ruminants. The ante mortem diagnosis of PTB is hampered, among other factors, by the limited sensitivity of all the available diagnostic techniques. Since TB-infected goats subjected to the comparative intradermal tuberculin test (CITT) may experience a booster effect on their antibody titer and a potential enhancement to the sensitivity of humoral techniques for tuberculosis, in the present study we aimed to evaluate this diagnostic strategy on the humoral diagnosis of PTB in serum and milk samples collected from a caprine herd that was TB free and PTB infected. The results from 120 goats indicated a significant … Leer más
Velasco-Reinaldos C., Ortega J., Gómez-Buendía A., Grau A., Lopez M., Alvarez J., Romero B., de Juan L. and Bezos J..