SCI scientific publications 2025
Reduction of Influenza A Virus Prevalence in Pigs at Weaning After Using Custom-Made Influenza Vaccines in the Breeding Herds of an Integrated Swine Farm System
Viruses-Basel 17(2):240.
Vaccination is a common influenza A virus (IAV) control strategy for pigs. Vaccine efficacy depends on strain cross-protection and effective vaccination program implementation. We evaluated a multi-faceted IAV vaccination strategy which included (a) monthly surveillance of pigs at weaning, (b) selection of epidemiologically relevant strains from farms under surveillance, (c) updating IAV strains in custom-made vaccines, and (d) seasonal mass vaccination with custom-made vaccines given to sows in 35 farrow-to-wean farms within an integrated swine farm system. Reduction of IAV in pigs from vaccinated sows was determined by monthly monitoring of farms for 30 months by IAV rRT-PCR (PCR) testing of nasal wipes collected from litters of piglets a… Leer más
Garrido Mantilla J., Sanhueza J., Alvarez J., Pittman JS., Davies P., Torremorel M. and Culhane MR.
Source attribution of human Campylobacter infection: a multi-country model in the European Union
Frontiers In Microbiology 16.
Introduction: Infections caused by Campylobacter spp. represent a severe threat to public health worldwide. National action plans have included source attribution studies as a way to quantify the contribution of specific sources and understand the dynamic of transmission of foodborne pathogens like Salmonella and Campylobacter. Such information is crucial for implementing targeted intervention. The aim of this study was to predict the sources of human campylobacteriosis cases across multiple countries using available whole-genome sequencing (WGS) data and explore the impact of data availability and sample size distribution in a multi-country source attribution model.
Methods: We constructed a machine-learning model using k-mer freque… Leer más
Thystrup C., Lykke-Brinch M., Henri C., Mughini-Gras L., Franz E., Wieczoreck K., Gutierrez M., Prendergast D., Duffy G., Burgess CM., Bolton D., Alvarez J., Lopez-Chavarrias V., Rosendal T., Clemente L., Amaro A., Zomer AL., Grimstrup-Joensen K., Moller-Nielsen E., Scavia G., Skarzynska M., Pinto M., Oleastro M., Cha W., Thepault A., Rivoal K., Denis M., Chemaly M. and Hald T.
Habitat suitability mapping and landscape connectivity analysis to predict African swine fever spread in wild boar populations: A focus on Northern Italy
PLoS ONE 20(1):e0317577.
African swine fever (ASF) is a highly contagious disease affecting wild and domestic pigs, characterised by severe haemorrhagic symptoms and high mortality rates. Originally confined to Sub-Saharan Africa, ASF virus genotype II has spread to Europe since 2014, mainly affecting Eastern Europe, and progressing through wild boar migrations and human action. In January 2022, the first case of ASF, due to genotype II, was reported in North-western Italy, in a wild boar carcass. Thereafter, numerous positive wild boars were identified, indicating an expanding wild epidemic, severely threatening Italian pig farming and trade. This study focused on the mapping of the suitable habitats for wild boars and their potential dispersal corridors in Northe… Leer más
Faustini G., Soret M., Defossez A., Bosch J., Conte A. and Tran A.
An Efficient, Organic Solvent-Free Method for Extraction and Concentration of Hepatitis E Virus from Pig Liver
Food Analytical Methods 1-9.
The presence of the hepatitis E virus (HEV) in pork products, particularly in pig liver has been frequently described. However, a standardized method is not still available for the detection of HEV in foods, particularly in those difficult food matrixes such as pig meat and pork products. The aim of this study was to design, optimize and evaluate a new method of food-separation and virus concentration for HEV in pig liver samples. This method is based on organic flocculation and avoids the use of organic solvents. The virus recovery rates and analytical sensitivity of the method using murine norovirus MNV-1 as a surrogate were 73.6–82.2% and at least 1 × 103 TCID50 per g of liver in 100% of the replicates, respectively. Furtherm… Leer más
Santamaria-Palacios J., Garcia N. and Rodriguez-Lazaro D.
Immunological mechanisms involved in the protection against development of pulmonary tuberculosis in naturally infected goats
Veterinary Microbiology 300:110320.
Tuberculosis (TB) is a notifiable zoonotic disease caused by bacteria of the Mycobacterium tuberculosis complex (MTBC) that affects a multitude of domestic and wild species. The main lesions caused by these mycobacteria are tuberculous granulomas, which determine the organism`s immune response to the disease. Although TB pathogenesis in cattle has been extensively studied, information regarding its progression in other species of interest for the maintenance and transmission of TB such as goats remains limited. This study aimed to characterise the immune response developed in the lungs of goats naturally infected with mycobacteria of MTBC by assessing key cell populations and immunomodulatory molecules involved in defending against TB. Henc… Leer más
Agullo-Ros I., Vaz-Rodrigues R., Dominguez M., Roy A., Ortega J., Moreno I., Bezos J., Dominguez L., Fernandez de Mera IG. and Risalde MA.
NpmC - a novel A1408 16S rRNA methyltransferase in the gut of humans and animals
International Journal Of Antimicrobial Agents 107382.
16S rRNA methyltransferases that act on residue A1408, NpmA and NpmB, confer high-level resistance to virtually all the aminoglycosides, but their reports are scarce. Analysing metagenomic projects in a One Health context, we identified in human and animal gut microbiomes from China and Canada a novel gene, npmC, that shares an identity of 91.5% with npmA, and up to 92.7% at amino acidic level. The protein encoded by this gene presents the conserved motifs required for A1408 methylation. Expression of the gene resulted in high-level of resistance to 4,5-disubstituted 2-deoxystreptamine (2-DOS) and to 4-monosubstituted 2-DOS aminoglycosides, as well as a moderate resistance to 4,6-disusbstituted 2-DOS aminoglycosides, including the last reso… Leer más
Matamoros BR., Serna-Bernaldo C., Wedel E., Montero N., Kirpekar F. and Gonzalez-Zorn B..