SCI scientific publications 2025
Sponge-based environmental DNA detection as a useful tool in monitoring Mycobacterium tuberculosis complex markers in European bison (Bison bonasus)
Scientific Reports 15(1):18503.
The European bison (Bison bonasus), also called wisent, is the largest terrestrial mammal in Europe, classified by the International Union for Conservation of Nature (IUCN) as a "Near Threatened" species. Tuberculosis (TB) represents a well-known threat to wisent, especially nowadays when infectious diseases are emerging to this species, due to locally high population density and frequent translocation (and consequently increased exposure to infectious diseases). There is an urgent need to control the TB-epidemiological situation in the European bison environment. This study aimed to assess the usefulness of the sponge-based environmental-DNA (eDNA) for monitoring TB in free-ranging and captive European bison herds based on the knowledge of… Leer más
Didkowska A., Perez-Sancho M., Herranz-Benito C., Klich D., Anusz K., Witkowski L., Dominguez L. and Gortazar C.
Diving Into the Depths: Unveiling the Main Etiologies of Piscine Lactococcosis With a Novel Multiplex qPCR Assay
Journal Of Fish Diseases e14147.
Piscine lactococcosis poses a significant threat to a wide range of cultured and wild fish populations worldwide, typically presenting as acute haemorrhagic septicemia with high morbidity and mortality. Although Lactococcus garvieae was historically considered the sole causative agent of piscine lactococcosis, recent studies have identified L. petauri and L. formosensis as additional, highly pathogenic species. In this study, we developed a novel TaqMan-based multiplex qPCR assay for the simultaneous detection and differentiation of L. garvieae, L. petauri and L. formosensis, following a pangenome analysis of the publicly available genomes of these bacterial species. The assay demonstrated high sensitivity and specificity across 156 bacteri… Leer más
Shahin K., Abdel-Glil M., Burcin Saticioglu I., Duman M., Altun S., Colussi S., Esposito G., Acutis PL., Marino P., Spondler B., Altinok I., Kotzamanidis C., Vela AI., Soto E., Gomes-Leal CA., Ajmi N. and Aoki S.
Mobile integrons encode phage defense systems
Science 388:6747.
Integrons are bacterial genetic elements that capture, stockpile, and modulate the expression of genes encoded in integron cassettes. Mobile integrons (MIs) are borne on plasmids, acting as a vehicle for hundreds of antimicrobial resistance genes among key pathogens. These elements also carry gene cassettes of unknown function (gcus) whose role and adaptive value remain unexplored. In this work, we show that gcus encode phage resistance systems, many of which are newly discovered. Bacteriophage resistance integron cassettes (BRiCs) can be combined and mixed with resistance cassettes to produce multiphage or drug and phage resistance. The fitness costs of BRiCs are variable and dependent on the genetic context and can be modulated by changin… Leer más
Kieffer N., Hipolito A., Ortiz-Miravalles L., Blanco P., Delobelle T., Vizuete P., Ojeda F., Jove T., Jurenas D., García-Quintanilla M., Carvalho A., Domingo-Calap P. and Escudero JA..
Evaluation of techniques for post-mortem diagnosis of Mycobacterium tuberculosis complex infection in goats
Veterinary Microbiology 304:110485.
Goats are highly susceptible to Mycobacterium tuberculosis complex (MTBC) infection and can play an important role in the epidemiology of animal tuberculosis (TB) in certain epidemiological scenarios. Historically, culture has been considered the only gold standard technique for post-mortem confirmation of MTBC infection. However, it is constrained by its low sensitivity, the slow growth of MTBC, and stringent biosecurity requirements. Thus, alternative post-mortem techniques are essential for effective TB control and eradication. This study aimed to compare the use of different diagnosis techniques for post-mortem confirmation of MTBC infection in goats positive for official tuberculin skin tests (TST). The techniques evaluated were direct… Leer más
Jimenez-Martin D., Munoz-Fernandez L., Agullo-Ros I., Jimenez-Pizarro N., Cano-Terriza D., Garcia-Bocanegra I., Romero B., Caballero-Gomez J., Gonzalvez M., Rejon S., Martinez R. and Risalde MA.
Reproductive loss attributed to Lactococcus petauri infection in a black-and-white ruffed lemur
Journal Of Veterinary Diagnostic Investigation 37(3):471-474.
Lactococci have been associated with fetal and neonatal infections in humans and cattle. Here we describe a case of reproductive loss attributed to Lactococcus petauri in a lemur. A full-term black-and-white ruffed lemur (Varecia variegata) was found dead in the indoor area of a zoologic exhibit. Classification as a late-term abortion or stillbirth was unclear as the precise gestational time was unknown. A medical checkup of the dam revealed fever and neutrophilic leukocytosis; recovery followed treatment with enrofloxacin. The main histologic findings were placental edema and hemorrhage, hepatic necrosis, desquamated amniotic epithelial cells in alveoli, and subendocardial and myocardial hemorrhages. Tissue Gram stain revealed abundant gra… Leer más
Rebollada A., Vela AI., Canales R., Romani-Cremaschi U., Ugarte-Ruiz M., Buendia A., Perez-Sancho M., Dominguez L., Fernandez-Garayzabal JF. and Rodriguez-Bertos A..
Trends and Challenges in the Detection and Environmental Surveillance of the Hepatitis E Virus
Microorganisms 13:998.
The Hepatitis E virus (HEV) is responsible for causing Hepatitis E, a zoonotic disease that has emerged as a significant global health concern, accounting for about 20 million infections and 70,000 deaths annually. Although it is often recognized as a disease that is acute in low-income countries, HEV has also been recognized as a zoonotic disease in high-income countries. The zoonotic transmission requires flexible approaches to effectively monitor the virus, vectors, and reservoirs. However, the environmental monitoring of HEV presents additional challenges due to limitations in current detection methods, making it difficult to accurately assess the global prevalence of the virus. These challenges hinder efforts to fully understand the sc… Leer más
Alves-Elois M., Paula-Pavi C., Ferreira Souza-Hoffmann Jempierre Y., Von Tönnemann-Pilati G., Zanchetta L., Borges-da Silva Grisard H., Garcia N., Rodriguez-Lazaro D. and Fongaro G.
Effect of oxygen on antimicrobial resistance genes from a one health perspective
Science Of The Total Environment 979:179523.
Bacteria must face and adapt to a variety of physicochemical conditions in the environment and during infection. A key condition is the concentration of dissolved oxygen, proportional to the partial pressure of oxygen (PO2), which is extremely variable among environmental biogeographical areas and also compartments of the human and animal body. Here, we sought to understand if the phenotype of resistance determinants commonly found in Enterobacterales can be influenced by oxygen pressure. To do so, we have compared the MIC in aerobic and anaerobic conditions of isogenic Escherichia coli strains containing 136 different resistance genes against 8 antibiotic families. Our results show a complex landscape of changes in the performance of resis… Leer más
Ortiz-Miravalles L., Prieto A., Kieffer N., Vergara E., Canton R., San Millan A., Baquero F., Hipolito A. and Escudero JA..
Oral fluid collection in wild boar: A field protocol
The Veterinary Journal 312:106362.
Traditional wildlife sampling methods have limitations in providing a well-distributed, timely and accurate disease surveillance in wildlife. We developed a field protocol for collecting wild boar (Sus scrofa) oral fluid samples for non-invasive active disease surveillance using environmental ropes. We deployed ropes in field settings and tested the rope-sampled oral fluids for antibodies against Mycobacterium bovis and closely related members of the Mycobacterium tuberculosis complex (MTC). Oral fluid samples were collected from three wild boars populations in Spain with endemic tuberculosis across two seasons with contrasting natural food availability. Additionally, we studied the effect of placing the ropes close to aggregation points an… Leer más
Relimpio D., Kosowska A., Barroso-Arevalo S., de Antonio Gomez DE., Gortazar C. and Barasona JA..
Revisiting Mycobacterium bovis SB0121 genetic diversity reflects the complexity behind bovine tuberculosis persistence in Spain
Preventive Veterinary Medicine 239:106519.
Identifying the causes of tuberculosis (TB) chronicity in cattle herds in Spain is a complex endeavour, mainly due to the multiple factors involved in persistence and the clonal population structure of the Mycobacterium tuberculosis complex. This study assessed the genomic diversity among M. bovis isolates belonging to SB0121, the most prevalent genotype in Spain, in chronically-infected herds. A total of 70?M. bovis isolates from 22 herds, located in six Spanish provinces, in which M. bovis SB0121 was isolated in at least three different sampling events were sequenced. Forty-three isolates from wildlife and cattle herds from the same or neighbouring municipalities to the problem herds were also included to identify putative local transmiss… Leer más
Lorente-Leal V., Pozo P., Bezos J., Collado S., Vicente J., Stuber T., Alvarez J., de Juan L. and Romero B..
Molecular epidemiology of Salmonella Enteritidis in humans and animals in Spain
Antimicrobial Agents And Chemotherapy 69(4):e0073824.
Salmonella Enteritidis, the most prevalent serovar causing human gastroenteritis, has been traditionally linked to poultry sources. Although antimicrobial resistance (AMR) is not common in this serovar, increasing levels of resistance to fluoroquinolones and ampicillin have been reported in 42 the last years. Here, 298 isolates retrieved from different sources (human, livestock, wildlife, food and
environment) and years (2002-2021) in Spain were analysed to evaluate their diversity, the distribution of AMR-conferring genes (ARGs) and mutations, and reconstruct the epidemiology of infection due to this serovar. Isolates were clustered in two major clades (I, II), with strains in clade I (including 61.5% of all human isolates) display… Leer más
Samper-Cativiela C., Torre-Fuentes L., Bernabé Diéguez B., Maex M., Ugarte-Ruiz M., Carrizo-Coronado P., Hernandez M., Hofle U., Saez-Llorente JL., De Frutos C., Aguero M., Moreno MA., Dominguez L., Herrera-Leon S. and Alvarez J..
Risk Factors and Long-Term Outcomes in Horses After the 2021 Outbreak of Equine Herpesvirus 1 Myeloencephalopathy, Valencia, Spain
Journal Of Veterinary Internal Medicine 39(2):e70040.
Background: Equine herpesvirus myeloencephalopathy (EHM) is a persistent threat to horses, with unclear risk factors and disease severity.
Objectives: To evaluate risk factors, effective reproduction rate (Rt), and long-term athletic outcomes of an EHM outbreak.
Methods: Retrospective study of the 2021 EHM outbreak in Valencia, Spain, examining associations between risk factors (sex, age, breed, country of origin, and vaccination status) and case fatality rate, EHM development, and odds of returning to competition using odds ratios [95% CI] and Rt via the Robert Kochs Institute method.
Results: Among 191 horses, 38 (20%) were clinically normal, 13 (7%) were subclinical, and 140 (73%) presented clinical signs (89 EHM, 64… Leer más
de la Cuesta-Torrado M., Velloso-Alvarez A., Santiago-Llorente I., Armengou L., Nieto F., Rios J., Cruz F. and Jose-Cunilleras E.
Exudative epidermitis by Staphylococcus hyicus producing ExhC: Control proposals against an emergent pathogen in intensive pig production
The Veterinary Journal 311:106338.
Exudative epidermitis (EE) is an occasional infectious disease affecting suckling and weaned piglets, with severe presentation in outbreaks. We described the diagnosis and control measurements conducted after an outbreak of EE caused by Staphylococcus hyicus producing an exfoliative toxin type C (ExhC) in a commercial 4000-head sow farm. Clinical disease was observed in suckling piglets, with a 70 % of morbidity, and a significant increase of mortality rate of 5 % during this period (p < 0.0001) was detected. The bacteria could be recovered from skin and internal organs of affected animals, and the pulsed-field gel electrophoresis (PFGE) analysis showed that all the isolates were genetically indistinguishable, suggesting a common origin. Ce… Leer más
Romero-Salmoral A., Alvarez-Delgado C., Munoz-Jimenez RA., Barraza P., Vela AI., Fernandez-Garayzabal JF., Gomez-Laguna J., Luque I. and Tarradas C.
Non-typhoidal Salmonella in food animals in Paraguay: predominant serovars and resistance phenotypes
Frontiers In Veterinary Science 12.
Surveillance of antimicrobial resistance (AMR) in Salmonella in livestock (poultry, pig, and cattle) is crucial to maintain food safety. Given the lack of information on the situation in livestock in Paraguay, the aim of this study was to determine the most frequent Salmonella serovars in poultry, pig and cattle sampled in slaughterhouses in the country in 2020–22 along with their AMR phenotypes using data from a national pilot program. Out of 1,161 samples collected from slaughtered animals originating from 189 farms nationwide, Salmonella was isolated from 91/384 (23.7%) samples from poultry, 52/390 (13.3%) from pigs and 6/387 (1.6%) from cattle. Seven serovars were identified in poultry, with Heidelberg being the most frequent (82.4% o… Leer más
Irrazabal R., Iriarte MV. and Alvarez J..
Malondialdehyde (MDA) and 8-hydroxy-2`-deoxyguanosine (8-OHdG) levels in canine serum: establishing reference intervals and influencing factors
BMC Veterinary Research 21:161.
Background
Mounting evidence suggests that malondialdehyde (MDA) and 8-hydroxy-2’-deoxyguanosine (8-OHdG) are valuable biomarkers of lipid and nucleic acid oxidation in numerous canine diseases. However, their application in clinical settings is limited due to the absence of reference intervals (RI) and the analytical inconsistencies. Therefore, this study aimed to characterize serum MDA and 8-OHdG concentrations in dogs, to establish assay-specific RI, and to identify biological, haematological and biochemical factors influencing these markers.
Methods
A total of 190 clinically healthy dogs were recruited, including pet dogs, working dogs and shelter dogs. Serum MDA concentration was measured by the Thiobarbituric Ac… Leer más
Perez-Montero B., Fermin-Rodriguez ML., Portero-Fuentes M., Sarquis J., Caceres S., Illera del Portal JC., de Juan L., Miro G. and Cruz F..
Integrative and conjugative elements associated with antimicrobial resistance in multidrug resistant Pasteurella multocida isolates from bovine respiratory disease (BRD)-affected animals in Spanish feedlots
The Veterinary Quarterly 45(1):1-15.
The emergence of multidrug-resistance (MDR) in Pasteurella multocida, a major contributor to bovine respiratory disease (BRD) is being increasingly reported, often linked to the carriage of antimicrobial resistance genes (ARGs) on integrative and conjugative elements (ICEs). The resistance phenotype for 19 antimicrobials was determined using broth microdilution in 75 Pasteurella multocida isolates from healthy and BRD-affected cattle from five feedlots. The genomes of 32 isolates were sequenced to identify ARG) and mobile genetic elements (MGEs) and assess their genetic diversity. MDR isolates (with phenotypic resistance to aminoglycosides, macrolides, fluoroquinolones and/or tetracyclines) were primarily found among BRD-affected compared t… Leer más
Serna C., Calderón-Bernal JM., Torre-Fuentes L., Garcia-Munoz A., Diez-Guerrier A., Hernandez M., Fernandez-Garayzabal JF., Vela AI., Cid D. and Alvarez J..
Genome Analysis of 6222 Bacterial Isolates from Livestock and Food Environments in Spain to Decipher the Antibiotic Resistome
Antibiotics 14:281.
Abstract: Background/Objectives: Antimicrobial resistance (AMR) poses a significant threat to global health and the economy, with projected costs ranging from $300 billion to $1 trillion annually and an estimated 10 million deaths per year by 2050. The food chain, from primary production to retail, represents a critical entry point for antimicrobial resistant bacteria into communities. This underscores the need for a coordinated “One Health” approach, integrating efforts in animal production, environmental health, and human healthcare to address this global concern. This study aimed to characterize the global resistome in Spanish primary production by sequencing 6222 bacterial genomes from animal origin. Methods and Results: Whole genom… Leer más
Hernandez M., Falco-Prieto A., Ugarte-Ruiz M., Miguela-Villoldo P., Ocampo-Sosa A., Abad D., Perez-Sancho M., Alvarez J., Dorighello-Cadamuro R., Alves-Elois M., Fongaro G., Quesada A., Gonzalez-Zorn B., Dominguez L., Eiros JM. and Rodriguez-Lazaro D.
Citizen Science to Raise Antimicrobial Resistance Awareness in the Community: The MicroMundo Project in Spain and Portugal
Microbial Biotechnology 18(3):e70123.
Antimicrobial resistance (AMR) poses a global threat to human, animal and environmental health. Among the multidisciplinary tasks aimed at collectively tackling the AMR crisis, surveillance, research and education stand as major priorities. Based on a crowdsourcing research strategy, the MicroMundo project, a partner of the Tiny Earth initiative in Spain and Portugal, has been developed and consolidated with success in the academic environment. The objectives are focused on promoting research and, especially, on bringing knowledge of One Health and microbiology concepts, as well as AMR awareness to the community. Following a service-learning approach, MicroMundo integrates university and secondary/high school students in a citizen science-b… Leer más
Gil-Serna J., Antunes P., Campoy S., Cid A., Cobo-Molinos A., Durao P., Fajardo C., Fouz B., Freitas AR., Grosso F., de Groot PWJ., de Miguel T., Gonzalez-Zorn B., Hinojosa B., Joao-Leao M., Llagostera M., de Llanos R., Lucia A., Maicas S., Marin I., Martinez-Canamero M., Miranda C., Molina-Guijarro JM., Moreno DA., de los Llanos-Palop M., Perez-Alvarez MJ., Pereira PM., Perez-Garcia MT., Quinteira S., Rioboo C., Robredo B., Rodriguez-Calleja JM., de la Haba RR., Sanchez S., Sanchez-Angulo M., Sanchez-Porro C., Sangari FJ., Santos B., Silveira E., Vicedo B. and Cid VJ.
Neurobrucellosis (Brucella ceti) in striped dolphins (Stenella coeruleoalba): Immunohistochemical studies on immune response and neuroinflammation
Veterinary Pathology 17:3009858241250336.
Neurobrucellosis is a shared condition of cetaceans and humans. However, the pathogenesis and immune response in cetacean neurobrucellosis has not been extensively studied. In this multicentric investigation, 21 striped dolphin (Stenella coeruleoalba) neurobrucellosis (Brucella ceti) cases diagnosed over a 10-year period (2012-2022) were retrospectively evaluated. For each case, morphological changes were assessed by evaluating 21 histological parameters. Furthermore, the immunohistochemical expression of Brucella antigen, glial fibrillary acid protein (GFAP), and a selection of inflammatory cell (IBA-1, CD3, and CD20) and cytokine (tumor necrosis factor-alpha [TNF-α], interferon-gamma [IFN-γ], interleukin [IL]-1β, IL-2, and … Leer más
Rebollada A., Giorda F., Pumarola M., Martino L., Gómez-Buendía A., Romani-Cremaschi U., Casalone C., Mattioda V., Di Nocera F., Lucifora G., Petrella A., Dominguez L., Domingo M., Grattarola C. and Rodriguez-Bertos A..
A Novel Prototype African Swine Fever Virus DIVA (Differentiation Between Infected and Vaccinated Animals) Serological Assay Based on the Detection of Antibodies Against the pEP153R, eGFP, and p72 Proteins
Vaccines 13:3.
Background/Objectives: African Swine Fever (ASF) is one of the most significant infectious diseases affecting both domestic pig and wild boar populations, leading to substantial economic and biosanitary consequences. In Europe, disease management relies on stringent biosecurity measures and surveillance through diagnosis, highlighting the urgent need for an effective and safe vaccine for ASF control. In this context, the VACDIVA project has generated several promising vaccine candidates, including those with the EP153R gene deleted and replaced by the eGFP reporter gene. Methods: In this study, pEP153R and eGFP proteins were produced using recombinant technology and demonstrated their antigenicity and DIVA capability through indirect ELISA.… Leer más
Gonzalez-Garcia G., Gallardo C., Monton M., Barroso-Arevalo S., Casado N., Barasona JA., Sanchez-Vizcaino JM., Venteo A., Sastre P. and Rueda P.
Reduction of Influenza A Virus Prevalence in Pigs at Weaning After Using Custom-Made Influenza Vaccines in the Breeding Herds of an Integrated Swine Farm System
Viruses-Basel 17(2):240.
Vaccination is a common influenza A virus (IAV) control strategy for pigs. Vaccine efficacy depends on strain cross-protection and effective vaccination program implementation. We evaluated a multi-faceted IAV vaccination strategy which included (a) monthly surveillance of pigs at weaning, (b) selection of epidemiologically relevant strains from farms under surveillance, (c) updating IAV strains in custom-made vaccines, and (d) seasonal mass vaccination with custom-made vaccines given to sows in 35 farrow-to-wean farms within an integrated swine farm system. Reduction of IAV in pigs from vaccinated sows was determined by monthly monitoring of farms for 30 months by IAV rRT-PCR (PCR) testing of nasal wipes collected from litters of piglets a… Leer más
Garrido Mantilla J., Sanhueza J., Alvarez J., Pittman JS., Davies P., Torremorel M. and Culhane MR.
Source attribution of human Campylobacter infection: a multi-country model in the European Union
Frontiers In Microbiology 16.
Introduction: Infections caused by Campylobacter spp. represent a severe threat to public health worldwide. National action plans have included source attribution studies as a way to quantify the contribution of specific sources and understand the dynamic of transmission of foodborne pathogens like Salmonella and Campylobacter. Such information is crucial for implementing targeted intervention. The aim of this study was to predict the sources of human campylobacteriosis cases across multiple countries using available whole-genome sequencing (WGS) data and explore the impact of data availability and sample size distribution in a multi-country source attribution model.
Methods: We constructed a machine-learning model using k-mer freque… Leer más
Thystrup C., Lykke-Brinch M., Henri C., Mughini-Gras L., Franz E., Wieczoreck K., Gutierrez M., Prendergast D., Duffy G., Burgess CM., Bolton D., Alvarez J., Lopez-Chavarrias V., Rosendal T., Clemente L., Amaro A., Zomer AL., Grimstrup-Joensen K., Moller-Nielsen E., Scavia G., Skarzynska M., Pinto M., Oleastro M., Cha W., Thepault A., Rivoal K., Denis M., Chemaly M. and Hald T.
Habitat suitability mapping and landscape connectivity analysis to predict African swine fever spread in wild boar populations: A focus on Northern Italy
PLoS ONE 20(1):e0317577.
African swine fever (ASF) is a highly contagious disease affecting wild and domestic pigs, characterised by severe haemorrhagic symptoms and high mortality rates. Originally confined to Sub-Saharan Africa, ASF virus genotype II has spread to Europe since 2014, mainly affecting Eastern Europe, and progressing through wild boar migrations and human action. In January 2022, the first case of ASF, due to genotype II, was reported in North-western Italy, in a wild boar carcass. Thereafter, numerous positive wild boars were identified, indicating an expanding wild epidemic, severely threatening Italian pig farming and trade. This study focused on the mapping of the suitable habitats for wild boars and their potential dispersal corridors in Northe… Leer más
Faustini G., Soret M., Defossez A., Bosch J., Conte A. and Tran A.
An Efficient, Organic Solvent-Free Method for Extraction and Concentration of Hepatitis E Virus from Pig Liver
Food Analytical Methods 1-9.
The presence of the hepatitis E virus (HEV) in pork products, particularly in pig liver has been frequently described. However, a standardized method is not still available for the detection of HEV in foods, particularly in those difficult food matrixes such as pig meat and pork products. The aim of this study was to design, optimize and evaluate a new method of food-separation and virus concentration for HEV in pig liver samples. This method is based on organic flocculation and avoids the use of organic solvents. The virus recovery rates and analytical sensitivity of the method using murine norovirus MNV-1 as a surrogate were 73.6–82.2% and at least 1 × 103 TCID50 per g of liver in 100% of the replicates, respectively. Furth… Leer más
Santamaria-Palacios J., Garcia N. and Rodriguez-Lazaro D.
High Frequency of Detection of NDM-Producing Enterobacterales Among Companion Animals Hospitalized in an Italian Veterinary Teaching Hospital
Transboundary And Emerging Diseases 2622185.
Carbapenems are considered one of the most important last-resort classes of antibiotics, and the spread of carbapenem-resistant Enterobacterales (CRE) is a serious concern worldwide. From a One Health point of view, reports on CRE in companion animals are increasing, requiring attention regarding their role in maintenance and direct transmission to humans. The aim of this study was to assess the frequency of detection at admission and the in-hospital acquisition of CRE from perirectal swabs in dogs and cats hospitalized in an Italian Veterinary Teaching Hospital (VTH). Of the 150 patients sampled, 11.3% (n?=?17) were CRE carriers at admission, 25.6% (n?=?34) acquired CRE in their commensal microbiota during their hospital stay, and 2% (n?=?… Leer más
Scarpellini R., Pulido-Vadillo M., Serna C., Gonzalez-Zorn B., Blanco JL., Delgado-Blas JF., Giunti M. and Piva S.
Immunological mechanisms involved in the protection against development of pulmonary tuberculosis in naturally infected goats
Veterinary Microbiology 300:110320.
Tuberculosis (TB) is a notifiable zoonotic disease caused by bacteria of the Mycobacterium tuberculosis complex (MTBC) that affects a multitude of domestic and wild species. The main lesions caused by these mycobacteria are tuberculous granulomas, which determine the organism`s immune response to the disease. Although TB pathogenesis in cattle has been extensively studied, information regarding its progression in other species of interest for the maintenance and transmission of TB such as goats remains limited. This study aimed to characterise the immune response developed in the lungs of goats naturally infected with mycobacteria of MTBC by assessing key cell populations and immunomodulatory molecules involved in defending against TB. Henc… Leer más
Agullo-Ros I., Vaz-Rodrigues R., Dominguez M., Roy A., Ortega J., Moreno I., Bezos J., Dominguez L., Fernandez de Mera IG. and Risalde MA.
NpmC - a novel A1408 16S rRNA methyltransferase in the gut of humans and animals
International Journal Of Antimicrobial Agents 107382.
16S rRNA methyltransferases that act on residue A1408, NpmA and NpmB, confer high-level resistance to virtually all the aminoglycosides, but their reports are scarce. Analysing metagenomic projects in a One Health context, we identified in human and animal gut microbiomes from China and Canada a novel gene, npmC, that shares an identity of 91.5% with npmA, and up to 92.7% at amino acidic level. The protein encoded by this gene presents the conserved motifs required for A1408 methylation. Expression of the gene resulted in high-level of resistance to 4,5-disubstituted 2-deoxystreptamine (2-DOS) and to 4-monosubstituted 2-DOS aminoglycosides, as well as a moderate resistance to 4,6-disusbstituted 2-DOS aminoglycosides, including the last reso… Leer más
Matamoros BR., Serna-Bernaldo C., Wedel E., Montero N., Kirpekar F. and Gonzalez-Zorn B..
